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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   2

Issue No. :  2

Year :  2009

Pages :   412-414

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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The Estimation of Aprepitant in Capsules Dosage forms by RP-HPLC.

Address:   V Kiran Kumar1, N Appala Raju2, Shabana Begum2, JVLN Seshagiri Rao3 and T Satyanarayana3
1Department of Pharmaceutical Chemistry, Sree Chaitanya Institute of Pharmaceutical Sciences, LMD.Colony, Karimnagar-505527.AP. 2Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road# 3, Banjara Hills, Hyderabad-500 034. #University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam,AP. *Corresponding Author

A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Aprepitant in Capsules dosage form. An X-Terra RP C18, 250x4.6 mm, 5 µm partical size, with mobile phase consisting of 0.01 M Ammonium Acetate containing 0.1% Formic acid and Methanol in the ratio of 35:65 v/v was used. The flow rate was 1.5 ml/min and the effluents were monitored at 210 nm. The retention time was 13.7 min. The detector response was linear in the concentration of 14-168 µg/ml. The respective linear regression equation being Y=267157.4x+169105. The limit of detection and limit of quantification was 0.28 and 0.84 µg/ml respectively. The percentage assay of Aprepitant was 99.85 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Aprepitant in bulk drug and in its pharmaceutical dosage form.
Aprepitant, RP-HPLC, Estimation, and Capsuless.
The Estimation of Aprepitant in Capsules Dosage forms by RP-HPLC. V Kiran Kumar, N Appala Raju, Shabana Begum, JVLN Seshagiri Rao, T Satyanarayana. Research J. Pharm. and Tech.2(2): April.-June.2009,;Page 412-414.
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