Journal :   Research Journal of Pharmacy and Technology

Volume No. :   2

Issue No. :  1

Year :  2009

Pages :   117-119

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Preparation and In vitro Evaluation of Hepatitis B Vaccine Encapsulated Chitosan Microparticles

Address:   SM Sivakumar1*, L Nirmala2, R Swarnalakshmi2, L Sivakumar2, B Anilbabu2 , TS Shanmugarajan2, J Anbu2, V Ravichandran2 and N Sukumaran3
1Divison of Research and Development, Nehru College of Pharmacy, Pampady, Thiruvilwamala – 680597, Kerala
2Department of Pharmaceutical Biotechnology, VEL’s College of Pharmacy, Pallavaram, Chennai
3Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412
*Corresponding Author

Adjuvant aimed to increase the immunogenicity of vaccines remain a focus in vaccine development. The purpose of this research work was to develop an alternative adjuvant for hepatitis B vaccine (HBsAg) to elicit a long lasting immune response after a single dose. The HBsAg encapsulated chitosan microparticles were prepared by emulsion cross linking technique. The newly developed microparticles were evaluated for vaccine content, particle size distribution and in vitro release study. The morphological characters were examined using scanning electron microscope (SEM). The release profile was investigated by the measurement of antigen in the releasing medium at various intervals. The microparticles were release antigen about 6 weeks in vitro. The results indicated that the chitosan polymeric microparticles showing sustained release of HBsAg with an initial burst effect.
Hepatitis B vaccine, Microparticles, Chitosan polymer, Sustained release.
SM Sivakumar, L Nirmala, R Swarnalakshmi, L Sivakumar, B Anilbabu, TS Shanmugarajan, J Anbu, V Ravichandran, N Sukumaran. Preparation and In vitro Evaluation of Hepatitis B Vaccine Encapsulated Chitosan Microparticles. Research J. Pharm. and Tech. 2(1): Jan.-Mar. 2009; Page 117-119.
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