Journal :   Research Journal of Pharmacy and Technology

Volume No. :   1

Issue No. :  4

Year :  2008

Pages :   366-369

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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A RP-HPLC Method for Simultaneous Estimation of Ambroxol Hydrochloride and Loratidine in Pharmaceutical Formulation



Address:   Krishna Veni Nagappan*, Meyyanathan SN, Rajinikanth B Raja, Suresh Reddy, Jeyaprakash MR, Arunadevi S Birajdar and Suresh Bhojraj
Department of Pharmaceutical Analysis, J S S College of Pharmacy, Rocklands, Ootacamund-643 001 Tamilnadu, India.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of Ambroxol Hydrochloride and Loratidine from pharmaceutical dosage forms. The method was carried out on a Phenomenex Gemini C18 (25 cm x 4.6 mm i.d., 5 ยต) column with a mobile phase consisting of acetonitrile: 50mM Ammonium Acetate (50:50 v/v) at a flow rate of 1.0 mL/min. Detection was carried out at 255 nm. Hydrochlorthiazide was used as an internal standard. The retention time of Ambroxol Hydrochloride, Loratidine and Hydrochlorthiazide was 5.419, 15.549 and 3.202 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can be used for the estimation of these drugs in combined Pharmaceutical dosage forms.
KEYWORDS:
Loratidine, Ambroxol Hydrochloride, RP HPLC and Pharmaceutical dosage form.
Cite:
Krishna Veni Nagappan, Meyyanathan SN, Rajinikanth B Raja, Suresh Reddy, Jeyaprakash MR, Arunadevi S Birajdar, Suresh Bhojraj. A RP-HPLC Method for Simultaneous Estimation of Ambroxol Hydrochloride and Loratidine in Pharmaceutical Formulation. Research J. Pharm. and Tech. 1(4): Oct.-Dec. 2008;Page 366-369.
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