Journal :   Research Journal of Pharmacy and Technology

Volume No. :   1

Issue No. :  3

Year :  2008

Pages :   215-217

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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A stability-indicating reverse phase high performance liquid chromatography method for the simultaneous determination of ramipril and valsartan in pharmaceutical dosage form



Address:   Sohan S. Chitlange1*, Mohammed Imran1, Kiran Bagri1 and DM Sakarkar2
1Pad. Dr. DY Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune- 18.
2S. N. Institute of Pharmacy, Pusad.
* Corresponding Author
DOI No:

ABSTRACT:
A reverse phase high performance liquid chromatography method for the simultaneous estimation of Ramipril (RAM) and Valsartan (VAL) in marketed formulation was developed. The determination was carried out on a Kromasil C18 (250 x 4.6 mm, 5µ m) column using a mobile phase of 0.05 M sodium perchlorate: acetonitrile: triethylamine (55: 45: 0.3% v/v, pH 3.0). The flow rate was 1.5 ml/min with detection at 211nm. The method was also applied for the determination of drugs in the presence of their degradation products. The retention time (RT) for ramipril was 5.03 min and for valsartan 9.07 min. Both the drugs showed linear response in the concentration range of 5-40µ g/ml. The correlation co-efficient (‘r’ value) for RAM and VAL was 0.9933 and 0.9976 respectively. The results of analysis have been validated statistically and by recovery studies. The % recoveries obtained for RAM and VAL ranges from 99.26 to 101.00%.
KEYWORDS:
Ramipril; Valsartan; Stability-indicating RP-HPLC.
Cite:
Sohan S. Chitlange, Mohammed Imran, Kiran Bagri, DM Sakarkar. A stability-indicating reverse phase high performance liquid chromatography method for the simultaneous determination of ramipril and valsartan in pharmaceutical dosage form. Research J. Pharm. and Tech. 1(3): July-Sept. 2008; Page215-217.
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